Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine.

نویسندگان

R E Thompson

C R Hewitt

D J Piper

W P Hansen

R H Rubin

N E Tolkoff-Rubin

M C Barrett

M J Nelles

چکیده

This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.

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